Abstract
The kinetically stable heptadentate gadolinium complex Gd.pDO3A (1.Gd) demonstrates significant 31P nuclear magnetic resonance (NMR) relaxation enhancement of biologically relevant phosphate species; adenosine triphosphate (ATP), phosphocreatine (PCr) and inorganic phosphate. Gd.pDO3A (1.Gd) binds these species in fast exchange, enabling the relaxation of the bulk phosphate species in solution. This gives rise to 31P relaxation enhancements up to 250-fold higher than those observed for 31P relaxation enhancements with the commercial MRI contrast agent Gd.DOTA (DOTAREM), 2. Gd.pDO3A-like complexes may have potential applications as 31P magnetic resonance contrast agents, since shortening the T1 relaxation time of phosphate species would reduce the time needed to acquire 31P-MR spectra.
Highlights
Lanthanide complexes are powerful tools for imaging and assay, and have played key roles in a variety of applications from contrast enhanced MRI1–4 to time-gated optical imaging and bioassay.[5,6,7]More than thirty years after the clinical approval of the first generation of MRI contrast agents, the clinical use of MRI contrast media remains broadly focused on the use of blood pool contrast agents.[15]
We describe how 31P nuclear magnetic resonance (NMR) methods can be used to probe the interaction of a lanthanide complex with some ionic phosphate species.[6,19,20,21]
The observed values of Ka are fully consistent with fast exchange between bulk phosphate and phosphate bound to 1
Summary
Lanthanide complexes are powerful tools for imaging and assay, and have played key roles in a variety of applications from contrast enhanced MRI1–4 to time-gated optical imaging and bioassay.[5,6,7]More than thirty years after the clinical approval of the first generation of MRI contrast agents, the clinical use of MRI contrast media remains broadly focused on the use of blood pool contrast agents.[15]. The kinetically stable heptadentate gadolinium complex Gd.pDO3A (1.Gd) demonstrates significant 31P nuclear magnetic resonance (NMR) relaxation enhancement of biologically relevant phosphate species; adenosine triphosphate (ATP), phosphocreatine (PCr) and inorganic phosphate.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
