Enhancement of the Yield of Bioethanol from Lignocellulose of Oat Hulls via Optimization of the Composition of the Nutritive Medium

Abstract

The studies were aimed at determination of the optimal composition of the nutritive medium to provide the maximal yield of bioethanol upon alcohol fermentation of the enzymatic hydrolyzate of lignocellulose material produced at the pilot scale via treatment of oat bran covering with diluted nitric acid. Biotechnological stages of saccharification and fermentation were achieved using available enzymatic agents CelloLux-A and BrewZyme BGX and ACCM (All-Russian Collection of Commercial Microorganisms) producer Saccharomyces сerevisiae Y-1693 that was stable to inhibiting action of hydrolyzates. The composition of the nutritive medium was determined to provide the yield of bioethanol as high as 89.9 % of the theoretical level that is 8.4 % higher of that in the presence of the native hydrolyzate. This nutritive medium contained 1.83 g/L ammonium sulfate, 0.98 g/L potassium monophosphate, 6.47 g/L yeastrel.