Abstract
Scenarios focus on the practical behavior of anaerobic decomposition systems to enhance biogas production, in addition to assure economic progression and ecological sustainability. The present study has framed to identify the potential hydrolytic bacteria from five different sources since principally the efficacy of hydrolytic bacteria determines the rate of hydrolysis of anaerobic decomposition and thereby biogas production. Among the 40 dominant bacteria isolated from diverse bases, 10 isolates were selected as efficient through preliminary screening. Consequently, the premier enzyme activity obtained from the isolate G5 obtained from goat rumen fluid for cellulase (44.16±1.00 U/ml), protease (260.63±1.35 U/ml) and lipase (33.20 ± 0.81 U/ml). Morphological, biochemical and molecular characterization revealed that G5 is Bacillus sp. DDG5 (KM093856.1). A range of pH (7.0-7.5) and temperature (40oC) was sufficient for the highest activity of hydrolytic enzymes experienced. Biogas production using cow dung showed an improved efficiency of 9.54 % in Bacillus sp. DDG5 treated tank (70.16 ± 1.54 %) in contrast to control (58.13 ± 1.02%) at 30th day. However, this study established that Bacillus sp. DDG5 obtained from goat rumen fluid is the promising hydrolytic bacteria, since it can be applied for proficient hydrolysis of various organic materials to enhance methane production in outlook.
 Int. J. Appl. Sci. Biotechnol. Vol 6(4): 386-396
Highlights
The reliance of fossil fuels has been restricted due to its nonrenewable nature, which surrogate biomass being the major contributor of energy generation in the upcoming scenarios by the rationale of increasing energy consumption
Different microbial groups are employed in respective stages and subsequently release biogas under anaerobic circumstances, where methane is accomplished as the flammable fraction (Divya et al, 2014)
The isolates were preliminary screened for the evaluation of major hydrolytic enzymes cellulase, protease and lipase on carboxyl methyl cellulose (CMC) agar, protease specific gelatin agar and tributyrin agar, respectively
Summary
The reliance of fossil fuels has been restricted due to its nonrenewable nature, which surrogate biomass being the major contributor of energy generation in the upcoming scenarios by the rationale of increasing energy consumption. Biogas production from biomass through anaerobic decomposition has great interest at the moment due to its renewable energy generation potential in addition to eco-friendly applications for greenhouse gas reduction, waste recycling and biofertilizer production. Different microbial groups are employed in respective stages and subsequently release biogas under anaerobic circumstances, where methane is accomplished as the flammable fraction (Divya et al, 2014). Among the four stages such as hydrolysis, acidogenesis, acetogenesis and methanogenesis were recognized in anaerobic
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