Enhancement of Serrapeptase Hyper Producing Mutant by Combined Chemical and UV Mutagenesis and its Potential for Fibrinolytic Activity

Shreya Gopinath,E. Selvarajan,K.N. Rajnish,Saptashwa Datta,R Venkataprasad

doi:10.22207/jpam.14.2.25

Abstract

Enhancement of Serrapeptase Hyper Producing Mutant by Combined Chemical and UV Mutagenesis and its Potential for Fibrinolytic Activity

Highlights

Enzymes are biocatalysts and act as therapeutic agents for many disorders related to metabolism[1]
A mutant with higher serrapeptase activity was developed by multiple exposures of Serratia marcescens to Ultra Violet radiation (UV) and Ethyl methyl sulfonate (EMS) individually and a combination of both the methods was used
After UV mutagenesis of S. marcescens for different time intervals of 5s, 10s, 20s, 30s, 40s, 50s, 60s, 5min, 10min, 20min, 30min, 60min using 15W power, the bacteria could survive up to 30min and the maximum activity of the UV treated enzyme was observed for 40sec exposure with an enzyme activity of 3234.9 EU/mL (Fig. 2A) which is higher than the wildtype Serrapeptase (2770 EU/mL) and is considered as the best UV irradiated mutant

Summary

Introduction

Enzymes are biocatalysts and act as therapeutic agents for many disorders related to metabolism[1]. Serrapeptase (EC number 3.4.24.40) is one of the proteolytic enzymes belonging to serine protease family that were used by the ancient Japanese biochemist about 25 years ago and is widely used in medicine in the Asian and European countries[5]. It has ability to dissolve only the dead proteins/nonliving tissues[6] and does not affect healthy tissues as the chemical structure of serrapeptase inhibits binding to proteins in healthy tissues[7]. It exhibits properties like antioedemic, analgesic, anti-inflammatory, dissolution of mucous, plaques and blood clots. Serrapeptase is effective in treating various diseases like atherosclerosis, arthritis, bronchitis, fibrocystic breast disease, crohn's disease, breast engorgement, Alzheimer disease, hepatitis, lung disorders, diabetes, and uterine fibroids[8]

Methods

Results

Discussion

Conclusion