Enhancement of Post-ischemic Myocardial Function by Chronic 17β-Estradiol Treatment: Role of Alterations in Glucose Metabolism

Abstract

This study was designed to assess the effects of chronic estrogen replacement therapy on mechanical function and glucose utilization in aerobic and post-ischemic hearts. Ovariectomized female rats were either untreated or were treated subcutaneously with 17β-estradiol (0.25 mg 21-day slow release pellets). After 14 days, when serum concentrations of 17β-estradiol were 3.8±0.8 and 148±15 pg/ml, respectively, hearts were isolated and perfused in working mode with Krebs–Henseleit solution containing 1.2 mmpalmitate and 11 mm[5-3H/U-14C]glucose. Hearts were perfused aerobically (60 min) and then subjected to low-flow ischemia (0.5 ml/min, 60 min) followed by reperfusion (30 min). During reperfusion, hearts from rats treated chronically with 17β-estradiol had an improved (two-fold) recovery of mechanical function. 17β-estradiol (400 pm, 109 pg/ml), when present acutely in heart perfusate during ischemia and reperfusion, did not improve recovery. Chronic 17β-estradiol increased glucose oxidation during reperfusion as well as during aerobic perfusion but had no effect on glycolysis. Chronic 17β-estradiol also altered post-ischemic glycogen metabolism and increased glycogen content and glycogen synthase activity at the end of reperfusion. As stimulation of glucose oxidation has been shown previously to be cardioprotective, and as the enhanced rate of glucose oxidation was not simply a consequence of enhanced recovery of mechanical function, alterations in glycogen and glucose utilization may contribute to the direct cardioprotective effects of chronic estrogen treatment.