Abstract
The bacterium, Enterobacter aerogenes NBO2 was cultivated in 250 ml Erlenmeyer flask under submerged fermentation and physiochemical characteristics were studied in order to improve the pectinase production. Fermentation condition improved were pH 6.5, cultivation temperature of 37°C, inoculums size of 3% (v/v) 7.4x10 8 cell/ml and agitation speed of 250 rpm, together with pectin concentration of 1.50% (w/v) and 0.26% of yeast extract as carbon and nitrogen sources, respectively had produced the highest pectinase production of about 18.54 U/mL and 0.43 g/L cell growth at 24 hours incubation time. The enzyme production by this bacterial isolate was found not growth dependent. There were increment in enzyme production and cell growth after improvement of physical and chemical parameters.
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