Abstract
Reticulitermes chinensis Snyder is a serious pest in China, and the odorant receptor co-receptor gene RcOrco plays a crucial role in olfaction. However, the function of RcOrco in the resistance of termites to entomopathogens has not been reported. We constructed dsRcOrco-HT115 engineered bacteria based on the RcOrco sequence from the full-length transcriptome data of R. chinensis. The engineered bacteria expressed dsRNA of RcOrco. Sonication was used to inactivate the dsRNA-HT115 strain and obtain a large amount of dsRcOrco. The dsRcOrco produced using this method overcame the problem that genetically engineered bacteria could not be applied directly and improved its effectiveness against termites. Bioassays using the dsRcOrco generated using this method showed that dsRcOrco significantly increased the toxicity of the bacterial and fungal pathogens to R. chinensis. The present study showed, for the first time, the function of Orco in termite resistance to pathogens, and the results provide a theoretical basis for the development and application of termite RNA biopesticides.
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