Abstract
Periodontitis is an inflammatory disease that leads to periodontal tissue destruction and bone resorption. Proliferation and differentiation of cells capable of differentiating into osteoblasts is important for reconstructing periodontal tissues destroyed by periodontitis. In this study, the effects of the nozone (no-ozone) cold plasma (NCP) treatment on osteoblastic differentiation in periodontal ligament (PDL) cells were investigated. To test the toxicity of NCP on PDL cells, various NCP treatment methods and durations were tested, and time-dependent cell proliferation was analyzed using a water-soluble tetrazolium salts-1 assay. To determine the effect of NCP on PDL cell differentiation, the cells were provided with osteogenic media immediately after an NCP treatment to induce differentiation; the cells were then analyzed using alkaline phosphatase (ALP) staining, an ALP activity assay, real time PCR, and Alizarin Red S staining. The NCP treatment without toxicity on PDL cells was the condition of 1-min NCP treatment immediately followed by the replacement with fresh media. NCP increased ALP, osteocalcin, osteonectin, and osteopontin expression, as well as mineralization nodule formation. NCP treatment promotes osteoblastic differentiation of PDL cells; therefore, it may be beneficial for treating periodontitis.
Highlights
Periodontitis is a local inflammatory disease characterized by the loss of periodontal tissues and alveolar bones [1]
The results showed that nificant changes in the expression β-catenin were observed after six hours of cell prowhile no significant changes in theofexpression of β-catenin were observed after six hours of cell proliferation and differentiation, after one day, 30 s of the NCP treatment led to an approximately 1.3-fold increase in β-catenin expression, while the 2 min NCP treatment sample showed an approximately 2.2-fold increase in β-catenin expression
When the differentiated cells were stained after two weeks, both the 30 s and 1 min NCP treatment samples showed increased staining intensity, and the alkaline phosphatase (ALP) activity was observed to have increased after the NCP treatment. These results indicate that the NCP treatment promoted the osteoblastic differentiation of the periodontal ligament (PDL) cells
Summary
Periodontitis is a local inflammatory disease characterized by the loss of periodontal tissues and alveolar bones [1]. Periodontal tissue destruction leads to tooth loss [2]. The fundamental goal of periodontal treatments is to apply reconstruction techniques to restore tissue structure and function. Recent studies have focused on tissue reconstruction or functional recovery after regeneration; of note are the studies on oral mesenchymal stem cells [3,4]. The periodontal ligament (PDL) is a specialized type of connective tissue that connects the surface of tooth roots to alveolar bones [5]. PDL cells are most abundant in the periodontal membrane, where they serve an important role in alveolar bone reconstruction [8]. Osteoblastic differentiation of PDL cells is essential for periodontal tissue reconstruction
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
