Abstract
Temporal focusing (TF) microscopy has a trade-off between temporal resolution, optical sectioning capability, and field of view. To improve this trade-off, we demonstrate time-multiplexed multi-line temporal focusing (TM-ML-TF) microscopy, which combines the time-multiplexed TF technique with multi-line focusing using a digital micromirror device. Compared with those for TF microscopy, the optical sectioning capability and the signal-to-background ratio were improved by factors of 2.5 and 4.17, respectively. In addition, by applying post-processing for structured illumination microscopy to TM-ML-TF microscopy, the optical sectioning capability and the signal-to-background ratio were further enhanced by factors of 1.2 and 4.63, respectively.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
