Abstract
Innovative removal of toxic nitrite and ammonia is one of the key approaches for efficient aquaculture biotechnology. This study suggests a fast removal technique of high-levels of nitrite and ammonia using recirculating aquaculture wastewater at 30‰ salinity. Four lab-scale moving bed biofilm reactors (MBBRs; R1, R2, R3, and R4) were operated in parallel for 56 days with different combinations of mature and fresh biofilms on biocarriers at 30% filling ratio. Nitrogen removal efficiency, biofilm physicochemical properties, and microbial analyses were conducted using PCR and sequencing techniques at the region of the 16S ribosomal RNA. The results showed that R4 (using mature biofilms) recorded the highest nitrite and ammonia removal (99.6% and 95.3%, respectively) compared to the control, R1 (94.1% and 89.4%). Meanwhile, the removal rate of ammonia in R4 was significantly the fastest compared to other reactors. Partial Least Squares-Discriminant Multivariate Analysis (PLS-DA) model showed a good prediction with very high accuracy and prediction (Q2 > 0.8) for the first three principal components pertaining to nitrite and ammonia removal. Planktosalinus genus was the most abundant in R4, while proteobacteria phylum was the dominant, followed by Marinobacter and Nitrospina in all reactors. Overall, the use of mature biofilms in MBBR was proven to increase bacterial diversity, enhancing nitrite and ammonia removal efficiency.
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