Abstract
Lycium chinense, a traditional Chinese herbal medicine is well known for its medicinal value and composition for centuries in Asia. Plant tissue culture technique has proven as a quick and sustainable ways to regenerate various plants species those are valuable in terms of medicinal as well as ornamental. Here, we investigated the in vitro root regeneration and root growth of L. chinense in response of different media, gelling agents and activated charcoal. The growth media Schenk and Hildebrandt medium (SH medium) performed the best for being the highest number of roots in each (3.50) and also for the longest root length (33.80 mm) exhibiting 20% and 18% higher roots/explant and root growth, respectively than that of the lowest roots/explant and root growth containg medium of MS. The highest number of roots (3.90) in each explant was observed when phytagar at 6 g L-1 has been used in the culture media producing a 26% higher number of roots in each explant. Phytagar at the lowest concentration (5 g L-1) used in this study produced the longest root length (35.10 mm) exhibiting 20% higher root length than that of the highest concentration (9 g L-1) of phytagar and after that with further increase the concentration of Phytagar the growth of the root length has been decreased. Here concentration of Gelrite at 3 g L-1 responded positively for getting the highest number of roots (4.00) in each explants and the longest root length (35.2 mm). Activated charcoal at 1 g L-1 produced the highest roots (4.10) in each explant and also enhanced to produce the longest (45.40 mm) root length these findings could contributed to enhance rooting ability in any crops especially some medical and ornamental cops and even could provide useful information for future industrial-scale root production of L. chinense.
Highlights
Lycium chinense is a shrub belongs to the Solanaceae family
Three different media i.e., SH, B5 and MS were used to investigate the variation of root regeneration as well their growth of Lycium chinense
Root regeneration and growth of root length significantly differed due to different growth media used in this study (Table 1)
Summary
Lycium chinense is a shrub belongs to the Solanaceae family. It is a prominent traditional Chinese herbal medicine which has a great variety of benefits especially for the immune modulating activity (Gan et al, 2004), anti-inflammatory effects (Oh et al, 2012), anti diabetic effects (Luo et al, 2004), anticancer properties (Mao et al, 2011) and anti-aging properties (Chang and So, 2008). The usual propagating system of any plants either tree, shrub or herb is from the seeds. Plants can be propagated with other vegetative ways. Regeneration through seeds needed much time and it is considered as low propagating methods of regeneration. Tissue culture techniques are just the opposite of traditional regeneration techniques where it is very applicable for clonal regeneration, genetically improvement and for the conservation of rare species
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