Abstract
Laccase is one of the enzymes suggested to play a role in fungal ligninolytic systems. Cotton stalks were found to be an excellent substrate for Pleurotus cultivation. In this work, the effects of cotton-stalk extract (CSE) were studied. It was found to stimulate both fungal growth in surface cultures and extracellular laccase activity in submerged cultures. Induction of laccase activity was dependent upon the concentration of the supplemented extract. The stimulation of laccase was observed as early as 3 h after amendment of CSE. Laccase activity staining after separation by gel electrophoresis revealed two major bands in both control and the CSE-induced cultures.
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