Abstract
Electrostatic forces at the cell interface affect the nature of cell adhesion and function; but there is still limited knowledge about the impact of positive or negative surface charges on cell-material interactions in regenerative medicine. Titanium surfaces with a variety of zeta potentials between −90 mV and +50 mV were generated by functionalizing them with amino polymers, extracellular matrix proteins/peptide motifs and polyelectrolyte multilayers. A significant enhancement of intracellular calcium mobilization was achieved on surfaces with a moderately positive (+1 to +10 mV) compared with a negative zeta potential (−90 to −3 mV). Dramatic losses of cell activity (membrane integrity, viability, proliferation, calcium mobilization) were observed on surfaces with a highly positive zeta potential (+50 mV). This systematic study indicates that cells do not prefer positive charges in general, merely moderately positive ones. The cell behavior of MG-63s could be correlated with the materials’ zeta potential; but not with water contact angle or surface free energy. Our findings present new insights and provide an essential knowledge for future applications in dental and orthopedic surgery.
Highlights
Bone defects due to fractures, infections or tumor resections are one of the main causes of disability in an aging society, leading to a loss of quality of life (Akter and Ibanez, 2016)
Amino Polymers Plasma polymerized allylamine (PPAAm) As it is known that the positively charged plasma polymerized allylamine (PPAAm) layer can improve several cell functions (Rebl et al, 2012; Mörke et al, 2017; Staehlke et al, 2018; Nebe et al, 2019), this plasma functionalization of Ti was used as a positive control in all experiments
plasma polymerized nanolayer of allylamine (PPAAm) films have proven to be very robust according to DIN EN 582 (1993), mechanically stable (Fritsche et al, 2009) and can be used for cell experiments for up to 200 days when stored in ambient air (Finke et al, 2014)
Summary
Bone defects due to fractures, infections or tumor resections are one of the main causes of disability in an aging society, leading to a loss of quality of life (Akter and Ibanez, 2016). Surface properties are one of the main factors influencing the cells’ fate by guiding cellular processes at the interface from the very beginning (Felgueiras et al, 2018; Ferrari et al, 2019). Initial processes driven by the surface properties involve cellular attachment, adhesion and spreading (von der Mark and Park, 2013), which further affect other cell activities such as proliferation, differentiation (Bacakova et al, 2011), and intracellular signaling (Anselme et al, 2010; Staehlke et al, 2015, 2018). Stimulating cellular behavior at the interface by acting on surface physico-chemical properties, especially roughness, stiffness, wettability, and surface charge via surface functionalization can be an effective way to improve bone regeneration (Ferrari et al, 2019)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
