Enhancement of industrial pectin production from sugar beet pulp by the integration of surfactants in ultrasound-assisted extraction followed by diafiltration/ultrafiltration

Abstract

To recover industrial pectin from sugar beet pulp (SBP), a combined surfactant and ultrasound-assisted extraction, followed by a concentration with ultrafiltration/diafiltration membranes is proposed. First, the operation conditions of the extraction were optimized for synthetic (Tween80, PEG4000) and natural (Saponin) surfactants. Tween 80 and Saponin provided maximum galacturonic acid (GalA) concentrations (5.5 and 5.8 g/L, respectively) under the best extraction conditions (4 gsurfactant/L, pH = 1, amplitude = 90%, and time = 90 min). The extracted liquid was purified and concentrated through membranes. The final retentate maintained 73% of the initial GalA and eliminated a high percentage of oligosaccharides. Finally, the pectin was precipitated with ethanol, and the precipitate solid contained 57 g GalA/100 g GalA of SBP with a high degree of esterification (DE = 81%) and MW (930 kDa). The pectin yield for the global process was 24.6%. The ultrasound-assisted extraction with surfactants followed by diafiltration/ultrafiltration could become a promising process for the chemical industry, able to provide pectin-enriched products of commercial interest from sugar beet pulp.