Abstract
Recombinant interferon preparations caused a dose-dependent increase of human monocyte cytotoxicity to the K562 and Daudi cell lines. Both rIFN-γ and rIFN-β enhanced this function to a similar extent, while rIFN- α c had less effect when compared on the basis of their anti-viral effects. Endotoxin and concanavalin A increased basal monocyte cytotoxicity while phagocytosis of latex particles had no effect. The increased monocyte cytotoxic effect of rIFN-β was completely abrogated by monoclonal antibody to IFN-β, while monoclonal antibody to IFN-γ had no effect. However, monoclonal antibody to IFN-γ only reduced the increased cytotoxic effect caused by rIFN-γ by 25%. Catalase inhibited both basal monocyte cytotoxicity and the increase in cytotoxicity following addition of rIFN-γ only slightly, suggesting that mechanisms other than the oxidative burst were active and could be induced by rIFN-γ.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
