Abstract
The aim of the present work is to investigate the effect of the He-Ne laser irradiation on the whole human blood (HB) in order to enhance the conditions of conservation. The HB was irradiated by He-Ne laser; (λ = 632 nm, continuous wave, power 30 mW, 2 mm diameter beam spot), electrical properties and complete blood count CBC were measured at three doses (0.0287, 0.0563 and 0.198 J/cm3) to the relevant best exposure dose during storage periods 9, 24, 30, 35 & 50 days. The irradiation process with the selected dose was performed by the exposure of the laser beam on the blood sample flow through narrow tube of cross section area, 0.0831 cm2. Blood dielectric parameters, (electric conductivity, dielectric constant, dielectric loss and dipole moment) and CBC, (red blood cell, white blood cell, hematocrit, hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin, and mean cell or corpuscular hemoglobin in concentration) were measured. The obtained results were compared with that of the control and showed that the best irradiation exposure dose suitable for increasing the time of blood storage with minimum changes in properties is 0.198 J/cm3 and storage period of about 50 days. The present study revealed that irradiation by He-Ne laser could be considered a good means to improve the conservation conditions of human blood.
Highlights
Biostimulation effects of laser irradiation on living organisms were found for three decades [1]-[4]
The aim of the present study is to find whether as a result of low level He-Ne laser action, the possibility to increase the viability of blood and get rejuvenation, in order to perform transfusion, as well as prolong its life preserved at 4 ̊C
In this study we had investigated the variation in human blood (HB) dielectric parameters (σ, ε', ε'', τ & D), due to laser irradiation as indication on the membrane structure and molecular motion
Summary
Biostimulation effects of laser irradiation on living organisms were found for three decades [1]-[4]. The study of the low level laser irradiation effects on blood is very important in the process of revealing the mechanisms of the action of laser radiation on biological tissues, as the blood is permanently composed of a diversity of cells whose membranes contains lipids, sugars and proteins. Blood plasma probably contains the most diverse range of biological products among all tissues, some being intrinsic to other organs (amino acids, proteins, lipids, hormones, antibodies and adjusting factors), exogenous substances or component substances (clotting and defense enzymatic systems, such as complement, etc.). Several clinical treatments with low-power laser irradiation have been applied to various pathologic processes, such as wound healing and tissue repair, as well as remodeling [9]-[12] play a more or less important role in achieving the biological effects [13]-[16]
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