Enhancement of high affinity γ-aminobutyric acid receptor binding in cerebellum of pyridoxine-deficient rat

Abstract

The high-affinity of [ 3H]γ-aminobutyric acid (GABA) to GABA A receptors and [ 3H]baclofen to GABA B receptors were studied in the cerebellum of pyridoxine-deficient rats and compared to pyridoxine-supplemented controls. There was a significant increase in the maximal binding (B max) of both GABA A and GABA B receptors with no significant difference in their binding affinities ( K d ). The changes observed suggest a supersensitivity of GABA A and GABA B receptors which seems to correlate negatively with the concentration of GABA in the cerebellum of pyridoxine-deficient rats.