Abstract
To study the regulation mechanisms of carp granulopoiesis, we injected Freund's complete adjuvant (FCA) into carp and measured the effect of FCA on (i) colony-stimulating activity (CSA) in serum, (ii) colony-forming cell response in hematopoietic tissues by using the semi-solid agar culture technique. Inlection of FCA caused a high level of serum CSA, which began to reise 1 day after injection. Peak activity occurred after 6 days, at which time CSA was 2.5-10 times higher than that of the pre-injection level. FCA-injection also increased the number of colony-forming cells in hematopoietic tissudes. When the hematopoietic cells obtained from non-injected carp were cultured, little or no colony formation was observed. But when the hematopoietic cells from FCA-injected carp were cultured, more than 40 colonies were formed. The colonies were mostly composed of basophilic granulocytes. A few neutrophilic granulocytes were also observed in the same colonies.
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