Abstract
BackgroundThis study aimed to validate the function of CKX gene on grain numbers in wheat.Methodswe constructed and transformed a RNA interference expression vector of TaCKX2.4 in bread wheat line NB1. Southern blotting analysis was used to select transgenic plants with single copy. The expression of TaCKX2.4 gene was estimated by Quantitative real-time PCR (qRT-PCR) analysis. Finally, the relation between expression of TaCKX2.4 gene and grain numbers was validated.ResultsTotally, 20 positive independent events were obtained. Homozygous lines from 5 events with a single copy of transformed gene each were selected to evaluate the expression of TaCKX2.4 and grain numbers per spike in T3 generation. Compared with the control NB1, the average grain numbers per spike significantly increased by 12.6%, 8.3%, 6.5% and 5.8% in the T3 lines JW39-3A, JW1-2B, JW1-1A and JW5-1A, respectively.ConclusionOur study indicated that the expression level of TaCKX2.4 was negatively correlated with the grain number per spike, indicating that the reduced expression of TaCKX2.4 increased grain numbers per spike in wheat.
Highlights
IntroductionThe plant hormone cytokinin (CK) promotes cell proliferation and differentiation, and regulates plant growth and development from many processes, such as senescence, shoot and root balance, transduction of nutritional signals, leaf senescence, chloroplast formation and crop productivity [1,2,3]
This study aimed to validate the function of Cytokinin oxidases/dehydrogenases (CKX) gene on grain numbers in wheat
In order to avoid the interference of endogenous genes in wheat, the presence of PiCKX2.4 vector in the putative transgenic plants was determined by genomic DNA Polymerase Chain Reaction (PCR) with the primers specific to FAD2 intron, a fragment of the interference vector
Summary
The plant hormone cytokinin (CK) promotes cell proliferation and differentiation, and regulates plant growth and development from many processes, such as senescence, shoot and root balance, transduction of nutritional signals, leaf senescence, chloroplast formation and crop productivity [1,2,3]. Stay-green can increase the yield of wheat and its resistance to heat stress during active photosynthesis [6]. Exogenous cytokinins can sustain longer active photosynthetic period during the grain filling stage, transfer more assimilates to the grain [7], and increase grain yield under heat stress [6]. The increase of photosynthetic productivity can lead to high yields through improvement of leaf anatomical and biochemical traits including tolerance to non-optimal temperature conditions [8]
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