Enhancement of ethylene biosynthesis and germination with thidiazuron and some selected auxins in Striga asiatica seeds

Abstract

Witchweed [Striga asiatica (L.) Kuntze], an economically important parasitic weed on several poaceous crops, is difficult to control. In nature, germination and subsequent morphogenesis of Striga are cued to specific host‐derived chemical signals. Seeds (approximately 2.4 mg) treated with thidiazuron (TDZ) or the auxins 2,4‐dichlorophenoxy‐acetic acid (2,4‐D), 1‐naphthalene acetic acid (NAA), or 2‐(4‐chloro‐o‐tolyloxy) propionic acid (MCPP) produced little ethylene (66‐138 nl l−1). Combinations of TDZ with the auxins increased ethylene production by 4‐ to 18‐fold. Ethylene production was strongly inhibited (86–92%) by aminoethoxyvinylglycine (AVG), inhibitor of 1‐aminocyclopropane‐1‐carboxylic acid (ACC) synthase. Ethylene evolved from seeds treated with TDZ in combination with 2,4‐D increased after a lag period and was promoted by a pretreatment in 2,4‐D. TDZ or any of the auxins, at the rates tested, effected negligible to low levels of germination (0 to 16%), whereas mixtures of TDZ with the above auxins stimulated 38 to 84% germination. Test solutions containing TDZ and indole‐3‐acetic acid (IAA) were, however, less effective. TDZ/auxin‐induced germination was inhibited by AVG and the ethylene action inhibitor silver thiosulfate (STS). The inhibitory effect of the former was reversed by treatment with ACC. In vitro studies revealed negligible germination (< 1%) on control medium. Seeds germinating on media containing TDZ alone developed into seedlings with distinct shoots and rudimentary roots. Seeds germinating on media containing 2,4‐D, irrespective of TDZ concentration, were induced to form calli. The results are consistent with a model in which both germination and subsequent morphogenesis in Striga are associated with exogenous and endogenous phytohormones.