Abstract
This study was to examine the effects of yeast extract (YE) and its fractions (YE1 and YE2) on the growth and diepoxin ζ (a spirobisnaphthalene with a diversity of bioactivities) production in liquid culture of Berkleasmium-like endophytic fungus Dzf12 from Dioscorea zingiberensis. When YE was applied to the liquid medium at 10 g/L on day 3 of culture, the diepoxin ζ production was most effectively enhanced 3.2-fold (378.70 mg/L versus 120.09 mg/L in control) after another 10 days culture. Feeding with 15 g/L of YE on day 9, the mycelia biomass reached 16.44 g/L, about 2.3-fold in comparison with the control (7.15 g/L). The polysaccharide fraction (YE1) was mainly responsible for stimulating diepoxin ζ accumulation, and the non-polysaccharide fraction (YE2) was mainly responsible for promoting mycelia growth. The results showed that the diepoxin ζ production in liquid culture of endophyte Dzf12 could be effectively enhanced by YE and its fractions.
Highlights
Plant endophytic fungi are an important and novel resource of natural bioactive compounds with great potential applications in agriculture, medicine and food industry [1,2,3,4]
The purpose of this study was to investigate the effects of yeast extract (YE) and its two fractions (i.e., YE1 as polysaccharide fraction, and YE2 as non-polysaccharide fraction) on the mycelia growth and diepoxin ζ production in liquid culture of Berkleasmium-like endophytic fungus Dzf12, a diepoxin ζ-producing endophytic fungus isolated from the rhizomes of a traditional Chinese medicinal plant Dioscorea zingiberensis C
The results indicated that day 13 was a suitable time for harvesting diepoxin ζ in liquid culture of endophyte Dzf12
Summary
Plant endophytic fungi are an important and novel resource of natural bioactive compounds with great potential applications in agriculture, medicine and food industry [1,2,3,4]. In the past two decades, many valuable bioactive compounds with antimicrobial, insecticidal, cytotoxic and anticancer activities have been successfully discovered from endophytic fungi. These bioactive compounds could be mainly classified as alkaloids, terpenoids, steroids, quinones, isocoumarins, lignans, phenylpropanoids, phenols and lactones [5,6,7,8,9]. Ogishi et al first reported a spirobisnaphthalene named MK 3018 from the fungus Tetraploa aristata [11].
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