Enhancement of diaminobenzidine colorimetric signal in immunoblotting.

Abstract

BioTechniquesVol. 23, No. 3 BenchmarksOpen AccessEnhancement of Diaminobenzidine Colorimetric Signal in ImmunoblottingLaurie A. Pukac, Jeffrey E. Carter, Kenneth S. Morrison & Morris J. KarnovskyLaurie A. Pukac*Address correspondence to Laurie Pukac, Harvard Medical School, Dept. of Pathology, 200 Longwood Ave., Boston, MA 02115-5701, USA. Internet: E-mail Address: lapuk@warren.med.harvard.eduHarvard Medical School, Boston, MA, USASearch for more papers by this author, Jeffrey E. CarterHarvard Medical School, Boston, MA, USASearch for more papers by this author, Kenneth S. MorrisonHarvard Medical School, Boston, MA, USASearch for more papers by this author & Morris J. KarnovskyHarvard Medical School, Boston, MA, USASearch for more papers by this authorPublished Online:7 Aug 2018https://doi.org/10.2144/97233bm08AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInRedditEmail FiguresReferencesRelatedDetailsCited ByA Highly Selective In Vitro JNK3 Inhibitor, FMU200, Restores Mitochondrial Membrane Potential and Reduces Oxidative Stress and Apoptosis in SH-SY5Y Cells2 April 2021 | International Journal of Molecular Sciences, Vol. 22, No. 7Sexual and ontogenetic variation of Bothrops leucurus venomToxicon, Vol. 184Detection and functional characterization of sigma class GST in Phlebotomus argentipes and its role in stress tolerance and DDT resistance23 December 2019 | Scientific Reports, Vol. 9, No. 1Optimization of von Willebrand factor multimer analysis in vertical mini-gel electrophoresis systems: A rapid procedureThrombosis Research, Vol. 175Expression of Recombinant PfCelTOS Antigen in the Chloroplast of Chlamydomonas reinhardtii and its Potential Use in Detection of Malaria30 November 2018 | Molecular Biotechnology, Vol. 61, No. 2Does the administration of pilocarpine prior to venom milking influence the composition of Micrurus corallinus venom?Journal of Proteomics, Vol. 174Examination of biochemical and biological activities of Bothrops jararaca (Serpentes: Viperidae; Wied-Neuwied 1824) snake venom after up to 54 years of storageToxicon, Vol. 141Optimization of non-denaturing protein extraction conditions for plant PPR proteins7 November 2017 | PLOS ONE, Vol. 12, No. 11Soluble Expression of Recombinant Nerve Growth Factor in Cytoplasm of Escherichia coli30 March 2016 | Iranian Journal of Biotechnology, Vol. 14, No. 1Ontogenetic Variation in Biological Activities of Venoms from Hybrids between Bothrops erythromelas and Bothrops neuwiedi Snakes29 December 2015 | PLOS ONE, Vol. 10, No. 12Antigen retrieval, blocking, detection and visualisation systems in immunohistochemistry: A review and practical evaluation of tyramide and rolling circle amplification systemsMethods, Vol. 70, No. 1Direct detection of rutin-degrading isozymes with polyacrylamide gel electrophoresisAnalytical Biochemistry, Vol. 443, No. 2Novel Secreted Antigens of Mycobacterium paratuberculosis as Serodiagnostic Biomarkers for Johne's Disease in CattleClinical and Vaccine Immunology, Vol. 20, No. 12In search of a tolerance-induction strategy for cow’s milk allergies: significant reduction of beta-lactoglobulin allergenicity via transglutaminase/cysteine polymerizationClinics, Vol. 67, No. 10Cerebellar oligodendroglial cells have a mesencephalic origin7 September 2011 | Glia, Vol. 59, No. 12Comparative analysis of newborn and adult Bothrops jararaca snake venomsToxicon, Vol. 56, No. 8Evaluation of albuminuria and its relationship with blood pressure in dogs with chronic kidney disease4 January 2010 | Veterinary Clinical Pathology, Vol. 39, No. 2Hydrogen peroxide- and nitric oxide-induced systemic antioxidant prime-like activity under NaCl-stress and stress-free conditions in citrus plantsJournal of Plant Physiology, Vol. 166, No. 17Bothrops jararaca fibrinogen and its resistance to hydrolysis evoked by snake venomsComparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, Vol. 151, No. 4Fabrication of Carbohydrate Microarrays on Gold Surfaces: Direct Attachment of Nonderivatized Oligosaccharides to Hydrazide-Modified Self-Assembled Monolayers16 June 2006 | Analytical Chemistry, Vol. 78, No. 14Polysaccharide−Poly(ethylene glycol) Star Copolymer as a Scaffold for the Production of Bioactive HydrogelsBiomacromolecules, Vol. 6, No. 4Simultaneous isolation of platelet factor 4 and glycoprotein IIb–IIIa complex from rabbit platelets, and characterization of specific chicken antibodies to assay themJournal of Immunological Methods, Vol. 284, No. 1-2Platelet-Derived Growth Factor-BB, Insulin-like Growth Factor-I, and Phorbol Ester Activate Different Signaling Pathways for Stimulation of Vascular Smooth Muscle Cell MigrationExperimental Cell Research, Vol. 242, No. 2 Vol. 23, No. 3 Follow us on social media for the latest updates Metrics Downloaded 632 times History Published online 7 August 2018 Published in print September 1997 Information© 2018 Author(s)PDF download