Enhancement of Cell Adhesion, Cell Growth, Wound Healing, and Oxidative Protection by Gelatins Extracted from Extrusion-Pretreated Tilapia (Oreochromis sp.) Fish Scale.

Chun-Yung Huang,Yong-Han Hong,Ren-Han Huang,Shu-Ling Hsieh,Tien-Chou Wu,Hui-Ru Guo

doi:10.3390/molecules23102406

Abstract

Gelatin has been broadly utilized in the food, pharmaceutical, photographic, cosmetic and packaging industries, and there is also huge potential for novel applications of gelatin in the fields of biotechnology and biomedicine. In the present study, we extracted gelatin from fish processing waste, i.e., scale of tilapia, by a combined method of extrusion-pretreatment and hot water extraction. The extrusion-pretreatment process increases the extraction yield of gelatin. Three gelatins (FS2: preconditioning with double-distilled water (ddH2O) before extrusion; FS12: preconditioning with citric acid solution before extrusion; FS14: preconditioning with acetic acid solution before extrusion) were obtained and all of them enhanced cell adhesion, cell growth, and wound healing in HaCaT cells and protected HaCaT cells from H2O2-induced cellular damage. Among FS2, FS12, and FS14, FS12 exhibited the most pronounced enhancement of cell adhesion, cell growth, and wound healing in HaCaT cells, and thus it may have potential as an effective natural raw material in cell therapies for cutaneous wounds and for reducing H2O2-induced oxidative damage of cells. In additional experiments, it was found that phosphorylations of Akt and mTOR are involved in the signaling pathway activated by FS2, FS12, and FS14 in HaCaT cells.

Highlights

Collagen is found in the extracellular matrix (ECM) of all eukaryotic tissues and one of its main functions is to provide physical protection of tissues [1]
Three gelatins FS2, FS12, and FS14 were produced according to the methods developed previously by our laboratory [24]
Different preconditioning processes were adopted for FS2, FS12, and FS14, the morphologies of these gelatins were not significantly

Summary

Introduction

Collagen is found in the extracellular matrix (ECM) of all eukaryotic tissues and one of its main functions is to provide physical protection of tissues [1]. A denatured type of collagen, can be dissolved in water and is often used as an ingredient for enhancing the functional properties of food products by augmenting their elasticity, consistency, and stability [5]. Gelatin is needed to make the outer shell of capsules in the pharmaceutical industry. Gelatin can be utilized as a binding and compounding agent in the manufacture of medicated tablets and pastilles. Gelatin is a promising agent as a plasma expander for blood in cases of harsh shock and injury [6]. Gelatin has a number of applications in the pharmaceutical industry [3,6], and there is huge potential for novel applications of gelatin in the fields of biotechnology and biomedicine

Methods

Results

Conclusion