Abstract
Bacillus clausii BS1 was coated by magnetic MFe3O4 nanoparticles (MNPs), and transmission electron microscope analysis of the cells showed that the MNPs were efficiently assembled and adsorbed on the surface of the microbial cell. The coated cells showed higher biodenitrogenation (BDN) rate toward CAR than that of the free cells, recording complete removal and 94.25% removal of 1000 ppm CAR within seven days, with t1/2 values of 31.36 and 64.78 h, respectively. Coated BS1 cells are characterized by higher BDN rate, storage and operational stabilities, and low sensitivity toward toxic by-products, and can be reused for four successive cycles without losing its biodenitrogenation efficiency and have the advantage of magnetic separation, which would resolve many operational problems in petroleum refinery.
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