Abstract

Black tea aroma is one of the essential attributes in determining the quality of black tea. β-Glucosidases were investigated for their ability to enhance the aroma of black tea by hydrolyzing the glycoside compound. The addition of β-glucosidase was done by dissolving the enzyme on a sodium citrate buffer (pH 5.0), which was then sprayed on tea leaves during black tea processing. The β-glucosidase treatment significantly increases the volatile compound from glycoside precursors such as linalool, geraniol, and methyl salicylate. Moreover, the volatile compound from carotenoid and lipid precursors (nerolidol and β-cyclocitral) was also increased with β-glucosidase treatment.

Highlights

  • Tea aroma is one of the essential properties which are decisive for the quality of the final product

  • The tea product aroma can be formed through various precursors such as carotenoids, fatty acids, terpenoids, phenylpropanoid/benzenoid, glycoside hydrolysis, and the Maillard reaction [1]

  • Aroma formation occurs due to oxidation and degradation of fatty acids and hydrolysis of glycosides [2]. cis-Jasmone and methyl jasmonate, which are derived from fatty acids, have fresh floral odors

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Summary

Introduction

Tea aroma is one of the essential properties which are decisive for the quality of the final product. The β-glucosidase enzyme could hydrolyze glycosides and release various free volatile compounds such as linalool and geraniol [6]. These compounds have a fruity floral odor and contribute significantly to black tea [7]. The concentration of methyl salicylate and Z-3-hexenol in steeping black tea increased by 2.14% and 8.24%, respectively, with the addition of the β-glucosidase enzyme [9]. It is unclear how β-glucosidase could improve the aroma of black tea during processing. The effect of adding the β-glucosidase enzyme, especially on aroma and other chemical components, during the black tea processing needs to be studied further

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