Enhancement of bacterial gene expression by insertion elements or by mutation in a CAP-cAMP binding site

Abstract

The regulatory region ( bglR) of the cryptic bgl operon was characterized by DNA sequence analysis and transcription mapping. Bgl −-specific transcription was found to occur in both the wild-type Bgl − and mutant Bgl + cells. However, the steady-state level of bgl RNA was much higher in the Bgl + mutant than in the wild-type. Activation of the bgl operon by insertion sequence-mediated bglR mutations or point mutations in bglR is therefore the result of increased transcription. The ethylmethane sulfonate-induced point mutations in bglR are alterations in a single base in the cAMP binding protein (CAP) binding site, leading to a stronger binding of the CAP-cAMP complex. The IS1 and IS5-mediated bglR mutations analyzed show that the insertion sequences can activate the bgl operon by integration 78 to 125 base-pairs upstream from the transcription initiation site. The role of the insertion sequences in activation of the bgl operon is discussed.