Abstract
A significant amount of research effort has been directed owards the enhancement of anthocyanin production in plant cell ulture. The present study analyses the possibility to combine and o integrate multiple productivity enhancement techniques (eliciation and two-stage culture systems) in a long-term Vitis vinifera . callus culture, acting synergistically to increase the anthocyanin roduction. The abiotic elicitors salycilic acid, mannitol, jasmonic cid, abscisic acid and ethephon have been used in a wide range f concentrations (10–40 (m) combined with culture filtrates of ungal origin (Fusarium oxysporum, Botrytis cinerea) as biotic eliciors. During the experiment pH differential methods were used for onomeric anthocyanin content determination and HPLC methds regarding quantitative and qualitative anthocyanin analyses. he obtained data suggested that a combined treatment for 31 days ith salicylic acid (10 (m) and jasmonic acid (40 (m) resulted in a ignificant synergistic enhancement of anthocyanin accumulation nour culture system. The current studydemonstrates that salycilic cid and jasmonic acid signaling pathways interact as integral parts f elicitor signal transduction leading to a significant anthocyanin ccumulation. oi:10.1016/j.copbio.2011.05.446
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