Abstract
The contents of anthocyanins, total glucosinolates, sulforaphane, and the metabolism of γ-aminobutyric acid (GABA) in broccoli sprouts grown under 1.0 mmol L−1 of mannose were investigated. The results showed that mannose treatment improved glutamate (Glu) content and the activity of diamine oxidase (DAO), and reduced γ-aminobutyric acid transaminase (GABA-T) activity, which enriched GABA biosynthesis. Mannose treatment enhanced the activities of diamine oxidase (DAO), polyamine oxidase (PAO), and 4-aminobutyraldehyde dehydrogenase (ABALDH), and increased the conversion rate of free polyamine. At the same time, it is also beneficial for the enrichment of anthocyanins, total glucosinolates and sulforaphane. These results indicated that mannose treatment has a significant enhancement effect on GABA and other health-promoting metabolites in germinated broccoli.
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