Abstract
Delayed-type hypersensitivity (DTH) to Rauscher murine leukaemia virus (R-MuLV) encoded or induced determinants was induced in mice by three syngeneic R-MuLV-induced tumour cell lines, i.e. a myeloid tumour, RMB-1, an erythroid tumour, RED-1, and a lymphoid tumour, RLD-1. DTH to subcutaneously (s.c.) administered RMB-1 cells appeared on day 4, with a maximum DTH response on day 6 or 7. The induction of DTH could be prevented by intravenous (i.v.) pre-immunisation with R-MuLV-induced tumour cells several days before the s.c. immunisation. The three R-MuLV-induced tumour cell lines showed cross-reactivity in the DTH assay, whereas no cross-reactivity was found with syngeneic WEHI-3 cells. This indicates that the three R-MuLV-induced tumour cell lines share a virally encoded or induced antigenic determinant, which activates T-cells. When the RMB-1 cells used for immunisation had been cultured in medium supplemented with interferon-gamma (IFN-gamma), the subsequent DTH response was increased. This coincided with an increased expression of the R-MuLV-specific antigenic determinants on RMB-1 cells as demonstrated by Scatchard analysis. Furthermore, IFN-gamma increased the MHC class I antigen expression on RMB-1 cells, whereas the class II antigen expression remained undetectable.
Highlights
Since sensitised helper T-cells produce macrophage attracting factors, they might play an important role in the activation and influx of macrophages in the tumour foci and subsequently in tumour destruction. Since such activities are characteristic for Delayed-type hypersensitivity (DTH) reactions, we studied whether Rauscher murine leukaemia virus (R-MuLV)-induced tumour cells induced DTH in syngeneic mice
After having established that R-MuLV-induced tumour cells evoke DTH, we investigated the specificity of this response, its suppression after i.v. pre-immunisation with irradiated tumour cells, and the effect of interferon-y (IFN-y) on the expression of the R-MuLV-induced tumour cell surface antigens and on the immunogenicity of RMB-1 cells as measured in the DTH assay
Induction of DTH reactivity to R-MuLV-induced tumour cells BALB/c responder mice were s.c. immunised with varying doses of syngeneic RMB-1 cells
Summary
BALB/c (H-2d) and DBA/2 (H-2d) female mice, 4 weeks of age, were purchased from Bomholtgard (Ry, Denmark). BALB/c (H-2d) female mice, 4 weeks of age, were purchased from Harlan Olac Ltd (Bicester, UK). Other BALB/c (H-2d) female and male mice were bred at the Department of Cell Biology, Immunology and Genetics of the Erasmus University. The WEHI-3 immature macrophage cell line of BALB/c origin, originally described by Warner et al (1969), was used as a control. This cell line does not express R-MuLV antigens. As a control culture supernatant from the CHO12RO cell line was used (Stefanini et al, 1982). When IFN-y was added to the tissue culture medium, it was supplemented to a final concentration of 100-150 IU ml- 1
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