Enhancement and statistical optimization (Plackett-Burman and response surface methodology) of biosurfactant production by marine bacteria Bacillus tequilensis KM15 using Fish processing wastes

Abstract

Biosurfactant (BS) production by Bacillus tequilensis KM15 is investigated using fish processing wastes as medium components. Bocha (B) and Rohu (RR) fish scales are selected as cost-effective substrates for BS production and optimized together with pH, the incubation period (IP), and inoculum size (IS) using the response surface method (RSM). RSM model has demonstrated the following optimum conditions for BS production: Bocha (B) and Rohu (RR) fish scales 4% each, and inoculum size 2%, at pH 8.0, 37 ̊C temperature in 48 h. A 2.8-fold increase in biosurfactant production was obtained (from 340 mg L-1 in basal medium to 944 mg L-1) using the response surface methodology. The major portion of the production cost can be reduced by using the currently studied substrates.