Abstract
Biovanillin production by a wild strain of Bacillus cereus NCIM-5727 is studied using eugenol as the precursor aiming to achieve maximum vanillin productivity. Based on shake flask optimization, molar yield and global volumetric productivity of vanillin reached up to 71.2% (6.6 gL-1) and 0.18g(Lh)-1, respectively, at 36h by resting cells of B. cereus NCIM-5727 at the optimum cell concentration of 3 gL-1 using eugenol concentration of 10 gL-1 at 37 ºC, buffer pH 7.0, buffer volume 10%, and shaking speed 180rpm. Furthermore, small-scale optimization in a bioreactor at the controlled aeration rate of 0.5 Lmin-1, agitation rate of 210rpm, and pH 7.0 enhanced the global volumetric productivity of vanillin up to 0.28g(Lh)-1 at 25h of bioconversion. The highest vanillin molar yield (75.2%) is reported using resting cells of B. cereus NCIM-5727 upon eugenol biotransformation and found stable for 10h.
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