Abstract
New CYP 119 T213G mutants were constructed and characterized. Introduction of T213G mutation into the wild-type CYP 119 enhances the turnover rate for the styrene epoxidation to 346.2 min−1, and the double T213G/T214V mutant improves the ratio of the S- and R-enantiomers of the epoxide products to 5.8. The molecular docking results support our initial design and experimental data.
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