Enhanced Treatment Effects of Tilmicosin Against Staphylococcus aureus Cow Mastitis by Self-Assembly Sodium Alginate-Chitosan Nanogel.

Kaixiang Zhou,Chao Li,Liwei Shao,Shuyu Xie,Shuge Wang,Qianying Liu,Yuanyuan Yan,Yuanyuan Yuan,Lingli Huang,Zonghui Yuan,Dongmei Chen,Xiaofang Wang

doi:10.3390/pharmaceutics11100524

Kaixiang Zhou, Chao Li + Show 10 more

Open Access

https://doi.org/10.3390/pharmaceutics11100524

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Abstract

The Staphylococcus aureus (S. aureus) cow mastitis causes great losses to the cow industry. In order to improve the treatment effect of tilmicosin against cow mastitis, the combination of solid lipid nanoparticle (SLN) technology with in situ hydrogel technology was used to prepare the self-assembly tilmicosin nanogel (TIL-nanogel). The physicochemical characteristics, in vitro release, antibacterial activity and in vivo treatment efficacy of TIL-SLNs and TIL-nanogel were studied, respectively. The results showed the loading capacity (LC), encapsulation efficiency (EE), size, zeta potential and poly dispersion index (PDI) of TIL-nanogel were 23.33 ± 0.77%, 67.89 ± 3.01%, 431.57 ± 12.87 nm, 8.3 ± 0.06 mv and, 0.424 ± 0.032, respectively. The TIL-nanogel showed stronger sustained release in vitro than TIL-SLNs and commercial injection. The cure rate of half dosage and normal dosage of TIL-nanogel was 58.3% and 75.0%, which was higher than that of commercial injection (50.0%) at normal dosage. The results suggest that the treatment dosage of tilmicosin for cow mastitis could be reduced by TIL-nanogel. The novel TIL-nanogel will be beneficial by decreasing the usage of tilmicosin and the treatment costs of cow mastitis.

Highlights

Bovine mastitis is a microbiology infectious inflammation of the mammary gland and one of the key and serious harmful pathogens is Staphylococcus aureus (S. aureus)
The results demonstrated that combination of TIL-solid lipid nanoparticle (SLN) with hydrogel (SA, CMCS) could improve the effect of tilmicosin against S. aureus mastitis
The results demonstrated that combination of TIL-SLNs with hydrogel (SA, CMCS) could improve Pthhaermeafcfeeuctticos 2f0t1il9m, 1i1c,o52s4in against S. aureus mastitis

Summary

Introduction

Bovine mastitis is a microbiology infectious inflammation of the mammary gland and one of the key and serious harmful pathogens is Staphylococcus aureus (S. aureus). It was reported that the proportion of Staphylococcal subclinical mastitis is 30% of cow mastitis [1]. The cow mastitis induced by S. aureus has led to several economic losses in cow industry, for example, the reduction of milk yield and quality, and an increase the slaughter rate and mortality of cows [2,3]. It was reported that the cost of mastitis treatment was about $200 per cow per year [4]. Because of the S. aureus infections, the loss of milk was nearly 380 tons per year in the world [5]. The situation of S. aureus bovine mastitis is severe and without available effective methods for its therapy

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