Abstract
The interaction of nandrolone decanoate drug dissolved in (2%) [BMIM]BF4 or [BMIM]PF6 with human serum albumin (HSA) at different temperatures in the range of 285-310 K was examined by fluorescence quenching. Stern-Volmer equation and its modified form were used to determine the interaction parameters K and n. The results revealed that binding affinities of HSA for nandrolone decanoate drug in 2% [BMIM]BF4 or [BMIM]PF6 are in the order of 105 M-1 and the number of bound drug molecules per HSA macromolecule are approximated to 1 at all temperatures studied. The thermodynamic parameters: free energy change (∆Go), enthalpy change (∆Ho) and entropy change (∆So) for HSA-nandrolone decanoate/ionic liquid were calculated according to van't Hoff equation. Data analysis indicated that both electrostatic interactions and hydrophobic ineractions played important roles in the interaction of nandrolone decanoate drug with HSA.
Highlights
The nature and types of interaction between small molecules and biomacromolecules characterize an active area of research
Nandrolone decanoate drug was selected as a model drug due to its limited solubility in water
The interaction between nandrolone decanoate and human serum albumin (HSA) in absence of ionic liquids showed very little quenching in fluorescence intensity which is an indication of marginal interaction
Summary
The nature and types of interaction between small molecules and biomacromolecules characterize an active area of research. Considering limited solubility of the drug in water, it is of interest to study the effect of some ionic liquids, namely 1-butyl-3-methyl-1-imidazolium tetrafluoroborate [BMIM][BF4] (Fig. 2.a) and 1-butyl-3-methyl-1-imidazolium hexafluorophosphate [BMIM][PF6] (Fig. 2.b) to increase its solubility in water and to enhance its ability to transfer the drug through the body Their behaviors were examined by following and analyzing the fluorescence spectra of human serum albumin-nandrolone decanoate mixture in the absence and presence of the selected ionic liquids as solvents. The fluorescence intensities were automatically recorded by injecting 20 μL samples of 80 μM drug solution containing 2% ionic liquids into the HSA solution which makes the final concentration of the test solution 0.148% considering the dilution effect. The inner-filter effect corrections[32] were done according to the formula:
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