Abstract
DNA transfection in cells is a key technique in biological studies. Cationic liposomes can form nanoparticle complexes with DNA and are widely used for gene delivery in mammalian cells. However, the major drawback of cationic liposomes is their low transfection efficiency in hard-to-transfect cells, such as primary cultured cells. In this study, we established a novel semi-attachment transfection method that showed remarkably improved transfection efficiency compared with traditional forward transfection method.
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