Abstract
BackgroundIt is well known that WRKY transcription factors play important roles in plant growth and development, defense regulation and stress responses.ResultsIn this study, a WRKY transcription factor, WRKY33, was cloned from Caragana korshinskii. A sequence structure analysis showed that it belonged to the Group-I type. Subcellular localization experiments in tobacco epidermal cells showed the presence of CkWRKY33 in the nucleus. Additionally, CkWRKY33 was overexpressed in Arabidopsis thaliana. A phenotypic investigation revealed that compared with wild-type plants, CkWRKY33-overexpressing transgenic plants had higher survival rates, as well as relative soluble sugar, proline and peroxidase contents, but lower malondialdehyde contents, following a drought stress treatment.ConclusionsThis suggested that the overexpression of CkWRKY33 led to an enhanced drought-stress tolerance in transgenic A. thaliana. Thus, CkWRKY33 may act as a positive regulator involved in the drought-stress responses in Caragana korshinskii.
Highlights
It is well known that WRKY transcription factors play important roles in plant growth and development, defense regulation and stress responses
The full-length cDNA of CkWRKY33 was obtained from total RNA extracted from drought-stressed C. korshinskii Caragana korshinskii Kom (Kom). leaves using RT-PCR
Sequence alignments between CkWRKY33 and other plant WRKY proteins indicated that the amino acid sequences of these proteins share a high similarity
Summary
It is well known that WRKY transcription factors play important roles in plant growth and development, defense regulation and stress responses. Plants have developed diverse adaptive mechanisms to respond to various abiotic stresses during the long-term evolutionary process [2]. Most of these mechanisms are controlled by networks regulated by transcription factors (TFs) [3]. TFs are proteins that can bind to cis-acting elements, WRKY TFs, which are named for their highly conserved WRKY domains, form a large family in higher plants and play important roles in many physiological processes [5, 6]. Group-I members generally contain two WRKY domains in the N-and C-terminal end, and its zinc finger structure
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