Abstract
Recently, we presented an improved procedure for the determination of fecal fat by means of Fourier transform infrared spectroscopy (1). This method can be used in laboratories equipped with a mid-infrared spectrometer. With this method, fecal fat was extracted from stool samples with petroleum ether-ethanol. After extraction, the petroleum ether was dried, and the fatty acids were redissolved in chloroform before measurement. Because the extraction procedure in the previously described analytical method (1) was still rather time-consuming, we replaced the petroleum ether-ethanol extraction with a single chloroform extraction. In the new extraction procedure, 1 g of homogenized …
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