Enhanced systemic resistance to bacterial speck disease caused by Pseudomonas syringae pv. tomato by dl‐β‐aminobutyric acid under salt stress

Abstract

Plants have evolved different but interconnected strategies to defend themselves against microbial pathogens and stress conditions. The defense responses of tomato (Lycopersicon esculentum Mill) seedlings treated with dl‐β‐aminobutyric acid (BABA) were investigated with and without abiotic stress (100 mM NaCl) against bacterial speck disease caused by Pseudomonas syringae pv. tomato. The plants were sprayed with 50, 125, 250 or 500 μg ml−1 BABA and were inoculated with 108 colony‐forming units ml−1 bacterial suspension 1 day after treatment. Abiotic stress led to an increase in plant resistance. When BABA was additionally applied as a foliar spray at 125 μg ml−1, the effect on plants was almost identical to that on plants that were sprayed with BABA at 500 μg ml−1 alone. The bacterial multiplication in the plants was 250‐fold lower than in the water‐treated (control) plants and in plants that were sprayed with 500 μg ml−1 BABA alone within 48 h postinoculation (hpi). Physiological studies were carried out in the plants treated with BABA in order to investigate the reason for this synergistic effect. Abiotic stress with BABA spray resulted in high H2O2 generation and guaiacol peroxidase activity in the plants. The activity of the enzymatic antioxidative protective system of the plants, superoxide dismutase, ascorbate peroxidase and catalase (CAT), also showed a significant delayed increase in BABA‐treated plants under abiotic stress conditions. These increases in enzyme activity coincided with the initiation of the most suppressive effect of BABA on bacterial growth by 24 hpi, which were significantly higher than the control. Salt stress alone did not lead to any significant increase in CAT activity, but salt stress with BABA did. These findings indicate a synergistic effect between salt stress and BABA at low concentrations, resulting in induced plant resistance. Furthermore, a stress regulation effect of BABA under abiotic stress can be associated with plant resistance.