Enhanced synthesis of andrographolide by Aspergillus niger and Penicillium expansum elicitors in cell suspension culture of Andrographis paniculata (Burm. f.) Nees.

Moinuddin M A Vakil,Vijay D Mendhulkar

doi:10.1186/1999-3110-54-49

Abstract

BackgroundAndrographis paniculata (Burm. f.) Nees is an important medicinal plant which has enormous applications in pharmaceutical industries. Cell suspension culture of Andrographis paniculata (Burm. f.) Nees. was treated with Aspergillus niger and Penicillium expansum elicitors to enhance the synthesis of andrographolide, the bioactive constituent of A. paniculata.ResultThe elicitation treatment with fungal elicitors (A. niger and P. expansum) was observed to be most suitable for eliciting andrographolide production in the culture. The quantification of andrographolide was done using High Performance Liquid Chromatography (HPLC) technique. A. niger extract (1.5 ml with10 days treatment duration) revealed 6.94 fold increase in andrographolide content (132 μg) which was higher than the control (19 μg). P. expansum elicitor (0.6% with 8 days treatment duration) could reveal 6.23 fold enhancement in andrographolide content (81.0 μg) over control (13 μg).ConclusionThe results obtained reveal that the longer treatment duration is most favorable for the elicitation of andrographolide using both the fungal elicitors.Electronic supplementary materialThe online version of this article (doi:10.1186/1999-3110-54-49) contains supplementary material, which is available to authorized users.

Highlights

Andrographis paniculata (Burm. f.) Nees is an important medicinal plant which has enormous applications in pharmaceutical industries
The results obtained reveal that the longer treatment duration is most favorable for the elicitation of andrographolide using both the fungal elicitors
High Performance Liquid Chromatography (HPLC) result A simple, isocratic, selective reversed phase-liquid chromatographic method was developed for the determination of andrographolide in the extracts of the cell suspension cultures of Andrographis paniculata (Burm. f.) Nees

Summary

Introduction

Andrographis paniculata (Burm. f.) Nees is an important medicinal plant which has enormous applications in pharmaceutical industries. F.) Nees is an important medicinal plant which has enormous applications in pharmaceutical industries. Plant cell culture is considered as a useful technique for the large scale production of plant secondary metabolites under in vitro level beside its utility for micropropagation. The production of these bioactive metabolites can be achieved by elicitation, cell immobilization, permeabilization (Brodelius et al 1989; Mendhulkar et al 2009), alteration in media composition etc. The plant is recommended for its drug utility in Indian Pharmacopoeia and widely used in Ayurveda, Unani, Siddha and Homeopathy systems of medicines.

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