Abstract
The stability of a 2μ-based recombinant plasmid, pJDB219, has been compared in glucose-limited chemostat cultures of two haploid strains ofSaccharomycescerevisiae and a diploid derived from them. The stability of the recombinant plasmid differed in the two haploid hosts but was greatest in the diploid. Enhanced stability in the diploid is probably a function of both the increased copy number and reduced selective burden of the plasmid.
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