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Abstract
Somatic embryogenesis is a preferred method for vegetative propagation due to its high propagation efficiency. In this study, zygotic embryos, cotyledons, and hypocotyls of Paeonia ostii ‘Fengdan’ were used as the explant to induce somatic embryogenesis. The results showed that a combination of 0.5 mg·L−1 thidiazuron (TDZ) and 0.5 mg·L−1 2,4-dichlorophenoxyacetic acid (2,4-D) was effective in inducing somatic embryos from the zygotic embryo and cotyledon explants. Hypocotyls only formed somatic embryos on Murashige and Skoog (MS) medium supplemented with both 0.5 mg·L−1 TDZ and 0.5 mg·L−1 1-naphthylacetic acid (NAA). Moreover, the compact callus was effectively produced from zygotic embryo, cotyledon, and hypocotyl explants in medium supplemented with a combination of 3.0 mg·L−1 6-benzylaminopurine (BA) and 1.0 mg·L−1 NAA, and then converted into somatic embryos in the same medium, and the ratio of the explants with embryo induction and number of embryos induced per explant were much higher than those induced by 0.5 mg·L−1 TDZ and either 0.5 mg·L−1 2,4-D or 0.5 mg·L−1 NAA. The MS medium was better than the woody plant medium (WPM) for inducing somatic embryos from zygotic embryo and hypocotyl explants, whereas the WPM was better than the MS medium for somatic embryogenesis induction from cotyledon explants. All of the somatic embryos developed well into mature embryos on their respective media supplemented with both 3.0 mg·L−1 BA and 1.0 mg·L−1 NAA. Overall, the protocols for indirect somatic embryogenesis from zygotic embryo, cotyledon, and hypocotyl of P. ostii ‘Fengdan’ were successfully established, which can greatly facilitate their propagation and breeding processes.
Highlights
Paeonia ostii ‘Fengdan’ is a wild tree peony species that belongs to Sect
Somatic embryos were successfully induced from the zygotic embryos, which were greatly affected by the combination of the basal medium and plant growth regulator (PGR)
The number of induced embryos per zygotic embryo was enhanced on the Murashige and Skoog (MS) medium supplemented with both 0.5 mg·L−1 2,4-D and either 0.5 mg·L−1 BA or 0.5 mg·L−1 TDZ, or on the woody plant medium (WPM) supplemented with both 0.5 mg·L−1 TDZ
Summary
Paeonia ostii ‘Fengdan’ is a wild tree peony species that belongs to Sect. Moutan DC., genus Paeonia, Paeoniaceae [1]. It is a well-known ornamental plant with perennial woody shrubs and large, ornate flowers, with more than 1500 years of history being cultivated [2]. It is a famous medicinal plant known for its root bark, known as cortexmoutan, which is an important ingredient in traditional. Seeds of P. ostii ‘Fengdan’ have recently attracted much attention, since it has a high oil content (27%) with more than 90% unsaturated fatty acids and more than 40%. The commercialization of cortexmoutan, peony seed oil, and other processed peony products causes an ever-increasing demand
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