Abstract
The sensitivity of thein vitro antibody-complement mediated dye exclusion cytotoxicity teat when carried out with rabbit antisera and mouse target cells was investigated with the polycation DEAE-dextran present in the cell suspension. One antiserum was raised against BALB/c mouse thymus cells (ATS) another against nude (nu/nu) mouse spleen cells (ANS). Target cells were from the spleens of normal BALB/c mice, cyclophosphatamide treated (B-cell depleted) BALB/c mice, and nude (T-cell depleted) mice. DEAE-dextran enhanced the sensitivity in all antiserum-target cell combinations, the enhancing effect being most pronounced when ANS was used against nude spleen cells.
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