Abstract
As a process for commercial application, production of reducing sugar, antioxidant, and DNA protective compounds from shrimp-shell powder was investigated in a fed-batch biodegradation using Bacillus cereus EW5. The fed-batch biodegradation was operated in a 5-L bioreactor for 96 h according to three times pulse-feeding strategy. On the basis of the equal working volume (3 L), the fed-batch biodegradation showed a better production of the target compounds than the batch biodegradation, with higher cell density and shortened biodegradation period. The maximum values of the target compounds were 0.297 mg/mL of reducing sugar, 92.35% DPPH radical scavenging activity, 98.16% ABTS radical scavenging activity, and 1.55 reducing power at A700, which were approximately 12.1, 3.4, 5.2, and 8.4% enhanced, respectively, compared with those obtained from the batch biodegradation. The fed-batch culture supernatant also showed the enhanced DNA damage inhibition activity than the batch culture supernatant. As a result, the fed-batch biodegradation accompanied by high cell density could produce more useful compounds, enabling an increase in the reutilization value of shrimp-shell waste.
Highlights
Shrimp-shell waste (SSW) is generated in huge quantities from the shrimp processing industries throughout the world, and it is primarily disposed of into the sea, causing intense environmental pollution (Suresh 2012)
The changes in kinetic parameters and the production of the bioactive compounds were compared with those obtained from the batch biodegradation to demonstrate the advantage of fed-batch biodegradation
A batch biodegradation was first executed, and a fed-batch biodegradation based on the growth characteristics of the batch biodegradation was followed to enhance the production of useful compounds from shrimp-shell powder (SSP)
Summary
Shrimp-shell waste (SSW) is generated in huge quantities from the shrimp processing industries throughout the world, and it is primarily disposed of into the sea, causing intense environmental pollution (Suresh 2012). Since this chitinous waste is considered as a valuable renewable resource, related studies have been carried out to convert it into useful compounds. Chitobiose, and GlcNAc were recovered by SSW biodegradation using the B. cereus EW5 (Azam et al 2014; Banik and Prakash 2004; Chen et al 2011; Ghorbel-Bellaaj et al 2012; Kim et al 2010; Talent and Gracy 1996; Wang et al 2009). Since competent production of the chitosaccharides from SSW has not yet reported, it is necessary to study the enhanced SSW biodegradation in a bioreactor level for commercial application
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