Enhanced removal of cephalexin and sulfadiazine in nitrifying membrane-aerated biofilm reactors

Abstract

Nitrification process has been reported to be capable of degrading various pharmaceuticals due to the cometabolism of ammonia-oxidizing bacteria (AOB). The membrane aerated biofilm reactor (MABR) is an emerging configuration in wastewater treatment with advantages of high nitrification rate and low energy consumption. However, there are very few studies investigating the degradation of antibiotics at environmentally relevant levels in nitrifying MABR systems. In this study, the removal of two widely used antibiotics, cephalexin (CFX) and sulfadiazine (SDZ), was evaluated in two independent MABRs with nitrifying biofilms. The impacts of CFX and SDZ exposure on the nitrification performance and microbial community structure within biofilms were also investigated. The results showed that nitrifying biofilms were very efficient in removing CFX (94.6%) and SDZ (75.4%) with an initial concentration of 100 μg/L when hydraulic retention time (HRT) was 4 h in the reactors. When HRT decreased from 4 h to 3 h, the removal rates of CFX and SDZ increased significantly from 23.4 ± 1.0 μg/(L·h) and 18.7 ± 1.1 μg/(L·h), respectively, to 27.7 ± 1.3 μg/(L·h) (p＜0.01) and 20.8 ± 2.4 μg/(L·h) (p＜0.05), while the removal efficiencies decreased to 86.0% and 61.5%, respectively. Despite the exposure to CFX and SDZ, the nitrification performance was not affected, and microbial community structure within biofilms also remained relatively stable. This study shows that nitrifying MABR process is a promising option for the efficient removal of antibiotics from domestic wastewater.