Abstract
Regardless of bacteria or eukaryotic microorganism hosts, improving their ability to express heterologous proteins is always a goal worthy of elaborate study. In addition to traditional methods including intracellular synthesis process regulation and extracellular environment optimization, some special or extreme conditions can also be employed to create an enhancing effect on heterologous protein production. In this review, we summarize some extreme environmental factors used for the improvement of heterologous protein expression, including low temperature, hypoxia, microgravity and high osmolality. The applications of these strategies are elaborated with examples of well-documented studies. We also demonstrated the confirmed or hypothetical mechanisms of environment stress affecting the host behaviors. In addition, multi-omics techniques driving the stress-responsive research for construction of efficient microbial cell factories are also prospected at the end.
Highlights
Production of recombinant proteins is a promising research field in many industries with particular requirements for biochemical active products, such as cosmetic industry, food industry and pharmaceutical industry
The advantages of low cultivation temperature include the favorable effects on energy metabolism, protein folding and secretion, protein degradation and aggregation in recombinant microorganism cells (Georgiou and Valax, 1996; Cassland and Jonsson, 1999; Li et al, 2001; Zepeda et al, 2018), on the economical efficiency in industrial fermentation production
The tool of omics has been frequently utilized to discover the impacts of environmental stress on metabolic activities of host cells, which could facilitate the improvement of recombinant protein production
Summary
Production of recombinant proteins is a promising research field in many industries with particular requirements for biochemical active products, such as cosmetic industry, food industry and pharmaceutical industry. Some stressful conditions including low temperature, hypoxia, microgravity and high osmolality are covered and their beneficial effects on heterologous protein expression in microorganism hosts are discussed extensively.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
