Enhanced RACE method using specific enrichment by biotinylated oligonucleotides bound to streptavidin coated magnetic particles.

S Lankiewicz

doi:10.1093/nar/25.10.2037

Enhanced RACE method using specific enrichment by biotinylated oligonucleotides bound to streptavidin coated magnetic particles.

S Lankiewicz

Open Access

https://doi.org/10.1093/nar/25.10.2037

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Journal: Nucleic acids research	Publication Date: May 15, 1997
Citations: 13	License type: implied-oa

Affiliation: Ruhr University Bochum

#Rapid Amplification Of cDNA Ends #Rapid Amplification Of cDNA Ends Method + Show 5 more

Abstract
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Abstract

RACE (rapid amplification of cDNA ends) is commonly used for identification and isolation of 3'and 5'termini of cDNA. We developed an improvement of the RACE-method that allows the enrichment of wanted fragments. The important new feature is the purification of the amplified products by biotinylated oligonucleotides that hybridize internally. Hybrids are isolated by streptavidin coated magnetic particles.

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