Abstract
Cell-encapsulated bioscaffold is a promising and novel method to allow fabrication of live functional organs for tissue engineering and regenerative medicine. However, traditional fabrication methods of 3D scaffolds and cell-laden hydrogels still face many difficulties and challenges. This study uses a newer 3D fabrication technique and the concept of recycling of an unutilized resource to fabricate a novel scaffold for bone tissue engineering. In this study, fish-extracted gelatin was incorporated with bioactive ceramic for bone tissue engineering, and with this we successfully fabricated a novel fish gelatin methacrylate (FG) polymer hydrogel mixed with strontium-doped calcium silicate powder (FGSr) 3D scaffold via photo-crosslinking. Our results indicated that the tensile strength of FGSr was almost 2.5-fold higher as compared to FG thus making it a better candidate for future clinical applications. The in-vitro assays illustrated that the FGSr scaffolds showed good biocompatibility with human Wharton jelly-derived mesenchymal stem cells (WJMSC), as well as enhancing the osteogenesis differentiation of WJMSC. The WJMSC-laden FGSr 3D scaffolds expressed a higher degree of alkaline phosphatase activity than those on cell-laden FG 3D scaffolds and this result was further proven with the subsequent calcium deposition results. Therefore, these results showed that 3D-printed cell-laden FGSr scaffolds had enhanced mechanical property and osteogenic-related behavior that made for a more suitable candidate for future clinical applications.
Highlights
Extensive bone tissue defects caused by trauma, surgical resection and bone diseases require complicated bone tissue repair and regeneration, but complete regeneration continues to be a great challenge in clinical medicine as bone tissues are known to have limited self-regenerating capabilities [1].Appl
FGSr scaffoldin showed that the wereinrather (Figure 1)
Photo imagewere of FGrather and FGSr scaffoldwith showed that the surfaces rather and smooth andThe that the struts continuous no excessive tears or were breakage atsmooth the middle that the struts were rather continuous with no excessive tears or breakage at the middle or the ends
Summary
Extensive bone tissue defects caused by trauma, surgical resection and bone diseases require complicated bone tissue repair and regeneration, but complete regeneration continues to be a great challenge in clinical medicine as bone tissues are known to have limited self-regenerating capabilities [1].Appl. Extensive bone tissue defects caused by trauma, surgical resection and bone diseases require complicated bone tissue repair and regeneration, but complete regeneration continues to be a great challenge in clinical medicine as bone tissues are known to have limited self-regenerating capabilities [1]. Autografts have some major drawbacks such as limited availability and invasive harvesting that requires additional surgery to harvest healthy bones from another site. Allograft is an alternative to bone repair; it is highly limited due to rejection and lack of availability. To solve this problem, researchers had attempted to develop novel biomaterials including ceramics, polymers and composites as an alternative choice for bone regeneration [3,4,5]. With the emergence of newer 3D printing techniques, we are able to fabricate scaffolds with customized shape, pore sizes and architectures that are similar to native bone [7,8]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
