Abstract
In the recent years, nattokinase is one of the most-often employed among the several thrombolytic agents used clinically, particularly because of its lower cost comparing to other thrombolytic agents. In the present research work, Bacillus sp. was isolated from the heterogeneous microbial population present in the soil sample and screened for the production of nattokinase. The production of the enzyme was carried out using two different media (with and without shrimp shell substrate). Nattokinase activity (clot buster) was determined by using a modified Holmstorm method. The production strain SFN01 was improved by random mutagenesis (UV radiation) and the enzyme activity was checked with the enzyme produced by wild strain. The mutated strains had exhibited a higher clot lysis activity in which 1 unit of the enzyme completely lyses 1 mL of human blood when compared to the wild strain. Nattokinase produced by SFN showed a retention time of 10.6 min in RP-HPLC chromatogram.
Highlights
Myocardial infarction is the most common contributor of morbidity and mortality worldwide
In this paper we report on the isolation and screening of nattokinase producing bacillus sp from soil
Our continuous search for Bacillus sp. from soil led to the isolation of one strain of Bacillus which was named as SFN (Search for nattokinase)
Summary
Myocardial infarction is the most common contributor of morbidity and mortality worldwide. The blood clots can detach from the vascular wall and travel in the blood These free floating thrombi ( called emboli) can lodge anywhere in the cardiovascular system, including the lungs or brain (as in a thrombotic stroke). The enzyme nattokinase offers a completely natural means of helping to prevent and dissolve blood clots. NK can cleave active recombinant prokaryotic plasminogen activator inhibitor into low molecular weight fragments (Urano et al, 2001) Those researches imply that NK is useful as a promising thrombolytic agent (Sumi et al, 1990). The nattokinase was extracted and precipitated for both normal and UV mutated Bacillus sp and the clot buster activity was studied
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