Enhanced production of l-serine by deleting sdaA combined with modifying and overexpressing serA in a mutant of Corynebacterium glutamicum SYPS-062 from sucrose

Abstract

The direct fermentative production of l-serine using Corynebacterium glutamicum from renewable biomass attracts increasing attention. This study was designed to enhance l-serine production in a wild-type C. glutamicum SYPS-062 by using random mutagenesis, optimization of media, and metabolic engineering. The resulting mutant, C. glutamicum SYPS 062-33a, accumulated 65.4% more l-serine (11.0±0.25g/L) and exhibited higher growth and production of other amino acids (such as l-alanine, l-valine) compared with the parent strain. Interestingly, this mutant harbors a better ability to consume sucrose, which is the main composition of economical molasses. To further enhance l-serine production, single sdaA deletion and serA modification was performed, which led to 14.76±0.82g/L and 19.42±1.20g/L l-serine, respectively. While double sdaA deletion and serA modification led to 21.27±1.87g/L l-serine. In addition, when the modified serA gene was overexpressed in C. glutamicum SYPS-062-33aΔSS, the resultant strain accumulated 24.43±0.95g/L and 21.6±1.2g/L l-serine in flask and 5-L bioreactor, respectively. Unlike the previous finding, this study demonstrated that the key to further enhance l-serine production may be the removal of the feedback inhibition of PGDH by l-serine in a high l-serine-producing strain C. glutamicum SYPS-062-33a.