Abstract
l(+)-Lactic acid production was investigated using 8 Rhizopus strains and various culture conditions. Rhizopus oryzae NRRL395 showed the highest yield of l(+)-lactic acid among various Rhizopus strains. The optimum production medium contained the following: corn starch, 120 g/ l; ammonium sulfate, 1.35 g/ l; small amounts of mineral salts (potassium phosphate, magnesium sulfate and zinc sulfate). The data obtained for the flask culture were successfully reproduced using a 3 l air-lift bioreactor under the optimum conditions. The yield (based on initial carbon source concentration) and final concentration of l(+)-lactic acid were 85% and 102 g/ l, respectively, in the 3 l air-lift bioreactor. The lactic acid was recovered with a yield (based on lactic acid containing in broth) of 90% using 4 steps, i.e., filtration, active carbon treatment, ultra-filtration and concentration by vacuum evaporation. The lactic acid purified was 100% l(+)-form as judged by HPLC.
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